Pepsin vs. Trypsin

By Jaxson

Main Difference

The main difference between Pepsin and Trypsin is that the Pepsin is a Enzyme and Trypsin is a family of digestive enzymes

  • Pepsin

    Pepsin is an endopeptidase that breaks down proteins into smaller peptides (that is, a protease). It is produced in the stomach and is one of the main digestive enzymes in the digestive systems of humans and many other animals, where it helps digest the proteins in food. Pepsin is an aspartic protease, using a catalytic aspartate in its active site.It is one of three principal proteases in the human digestive system, the other two being chymotrypsin and trypsin. During the process of digestion, these enzymes, each of which is specialized in severing links between particular types of amino acids, collaborate to break down dietary proteins into their components, i.e., peptides and amino acids, which can be readily absorbed by the small intestine. Pepsin is most efficient in cleaving peptide bonds between hydrophobic and preferably aromatic amino acids such as phenylalanine, tryptophan, and tyrosine.Pepsin’s proenzyme, pepsinogen, is released by the chief cells in the stomach wall, and upon mixing with the hydrochloric acid of the gastric juice, pepsinogen activates to become pepsin.

  • Trypsin

    Trypsin (EC is a serine protease from the PA clan superfamily, found in the digestive system of many vertebrates, where it hydrolyzes proteins. Trypsin is formed in the small intestine when its proenzyme form, the trypsinogen produced by the pancreas, is activated. Trypsin cuts peptide chains mainly at the carboxyl side of the amino acids lysine or arginine. It is used for numerous biotechnological processes. The process is commonly referred to as trypsin proteolysis or trypsinisation, and proteins that have been digested/treated with trypsin are said to have been trypsinized. Trypsin was discovered in 1876 by Wilhelm K├╝hne and was named from the Ancient Greek word for rubbing since it was first isolated by rubbing the pancreas with glycerin.

  • Pepsin (noun)

    A digestive enzyme that chemically digests, or breaks down, proteins into shorter chains of amino acids.

  • Trypsin (noun)

    A digestive enzyme that cleaves peptide bonds (a serine protease)


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